Chemistry Department

Saint Mary's College of California

   
  

 
 

 

Jeffrey A. Sigman, Ph.D.

 

Saint Mary’s College of California
Department of Chemistry
Moraga, CA  94575-4527

www.jsigman.alinbiq.org
jsigman@stmarys-ca.edu
(925) 268-0970


Professional Training


Ph.D. Bioinorganic Chemistry
University of Illinois (2000)
Advisor: Prof. Yi Lu
Thesis: “Engineering Metalloproteins: Design and Characterization of Metal-binding Sites in Cytochrome c Peroxidase and Myoglobin”

M.S. Chemistry
St. Louis University  (1993)

B.S. Chemistry
St. Louis University (1991)

 

Teaching appointments

 

Professor of Chemistry
Saint Mary’s College of California (2010-present)

Associate Professor of Chemistry
Saint Mary’s College of California (2005-2010)

Assistant Professor of Chemistry
Saint Mary’s College of California (2003-2005)

Visiting Professor of Chemistry
Wellesley College (2002-2003)

Camille and Henry Dreyfus Teaching/Research Fellow
Wellesley College (2000-2002)

 
 

Teaching experience

 

Most recent courses designed, prepped, and taught

  • Introductory Chemistry I (2011)
  • Introductory and Advanced Biochemistry Lecture and Lab (2012)
  • General, Organic, Biochemistry (for Health Sciences) Lecture and Lab (2011)
  • Advanced Inorganic Chemistry (2010)
  • Bioinorganic Chemistry Seminar (2007)
 

Most recent courses prepped and taught

  • Introductory Chemistry I Lab (2011)
  • Introductory Chemistry II Lab (2012)
  • Collegiate Seminar (2012)
 

Course materials produced

  • Authored student-led group problems and process-oriented guided inquiry learning (POGIL) worksheets (published under Creative Commons attribution license)
  • Produced online instructional videos to support inorganic and general chemistry courses
  • Authored multiple HTML/CSS and MySQL/PHP (Joomla!) course websites
 

Student research mentorship

  • Mentored undergraduate researchers for summer projects and independent study projects in protein biochemistry
  • 14 students at Saint Mary’s College of California (2003-2012)
  • 7 students at Wellesley College (2000-2003)
  • 2 students, Lori Scognamillo and Danica Randall, won recognition for their work by receiving honorable mention awards at the American Society for Biochemistry and Molecular Biology undergraduate research poster competitions (2006, 2009).
 
 

OS and iOS application skills

 

Significant experience

  • Joomla!
  • Microsoft Office
  • Adobe Dreamweaver
  • Adobe Contribute
  • Adobe Fireworks
  • Adobe Acrobat Pro

  • Papers
  • Swiss PDBviewer
  • Kaleidagraph
  • Camtasia
  • Doceri
  • Evernote
 
 

Moderate experience

  • Adobe Flash
  • Photoshop
  • SigmaPlot
  • Google Docs

  • Pymol
  • Jmol
  • Blackboard
  • Moodle
 
 
 

Rich and multimedia authorship

 

Joomla MySQL/PHP websites/content management systems

 
  • http://www.facultytechnology.org. Faculty Technology Group website and content management system. A faculty development resource providing documentation and resources to faculty incorporating technology into teaching and research. Includes an online grant application, user reporting function, and a discussion board for committee evaluation of grants.
  • http://www.biochem.alinbiq.org. Website and content management system to support advanced biochemistry lecture and lab. Included syllabus, news feeds, links, assignments, discussion board, and multimedia resources.
  • http://www.inorganic.alinbiq.org. Website and content management system to support advanced inorganic chemistry lecture. Included syllabus, news feeds, links, assignments, discussion board, and multimedia resources.

HTML/CSS websites (selected examples)

Video (selected examples)

 
 

Affiliations

 

American Society for Biochemistry and Molecular Biology (ASBMB)

 
 

Publications                                                      

(bold indicates undergraduate authors)

 
  • Bruce, L., Sigman, J., Randall, D., Rodriguez, S., Song, M., Dai, Y., Elmore, D., Pabon, Amanda; Glucksman, M., Wolfson, A. (2008) Hydrogen bond residue positioning in the 599-611 loop of thimet oligopeptidase is required for substrate selection. FEBS J. 275 (22), 5607-17.
  • Sigman, J., Patwa, T., Tablante, A., Joseph, C., Glucksman, M., Wolfson, A. (2005) Flexibility in substrate recognition by thimet oligopeptidase as revealed by denaturation studies. Biochem. J. 388, 255-61.
  • Sigman, J. (2004) Thermodynamic properties of peptide folding. Biochemistry and Molecular Biology Education 32, 265-268
  • Sigman, J., Edwards, S., Pabon, A., Glucksman, M., Wolfson, A. (2003) pH dependence studies provide insight into the structure and mechanism of thimet oligopeptidase (EC 3.4.24.15). FEBS Lett. 545 (2-3), 224-8.
  • Sigman, J., Sharky, M., Walsh, S., Pabon, A., Glucksman, M., Wolfson, A. (2003) Involvement of surface cysteines in activity and multimer formation of thimet oligopeptidase. Protein Eng. 16 (8), 623-8.
  • Perera, R., Sono, M., Sigman, J., Pfister, T., Lu, Y., Dawson, J. (2003) Neutral thiol as a proximal ligand to ferrous heme iron: implications for heme proteins that lose cysteine thiolate ligation on reduction. Proc. Natl. Acad. Sci. U.S.A. 100 (7), 3641-6.
  • Sigman, J., Kim, H., Zhao, X., Carey, J., Lu, Y. (2003) The role of copper and protons in heme-copper oxidases: kinetic study of an engineered heme-copper center in myoglobin. Proc. Natl. Acad. Sci. U.S.A. 100 (7), 3629-34.
  • Sigman, J., Wang, X., Lu, Y. (2001) Coupled oxidation of heme by myoglobin is mediated by exogenous peroxide. J. Am. Chem. Soc. 123 (28), 6945-6.
  • Sigman, J., Kwok, B., Lu, Y. (2000) From myoglobin to heme-copper oxidase: Design and engineering of a CuB center into sperm whale myoglobin. J. Am. Chem. Soc.122 (34), 8192-8196.
  • Sigman, J., Kwok, B., Gengenbach, A., Lu, Y. (1999) Design and Creation of a Cu(II)-Binding Site in Cytochrome c Peroxidase that Mimics the CuB-heme Center in Terminal Oxidases. J. Am. Chem. Soc. 121, 8949-8950.
  • Sigman, J., Pond, A., Dawson, J., Lu, Y. (1999) Engineering cytochrome c peroxidase into cytochrome P450: a proximal effect on heme-thiolate ligation. Biochemistry 38 (34), 11122-9.
  • Yeung, B., Wang, X., Sigman, J., Petillo, P., Lu, Y. (1997) Construction and characterization of a manganese-binding site in cytochrome c peroxidase: towards a novel manganese peroxidase. Chem. & Biol. 4 (3), 215-21.
 
 

Poster presentations          

(bold indicates undergraduate authors and presenters)

 
  • Pratini, Napala R., Shaw, B., Sigman, Jeffrey A., and Glucksman, Marc J. “The effect of calcium on conformational change of thimet oligopeptidase" American Society for Biochemistry and Molecular Biology Annual Meeting, Washington DC, 2011.
  • Lin, Zhe J., Sigman, Jeffrey A., and Glucksman, Marc J. “Conformational change analysis of thimet oligopeptidase” American Society for Biochemistry and Molecular Biology Annual Meeting, Washington DC, 2011.
  • Scognamillo, Lori A., Sigman, Jeffrey A., Glucksman, Marc J., Pabon, A., Wolfson, Adele J. "Evidence for a conformational change in thimet oligopeptidase and the machinery behind it" American Society for Biochemistry and Molecular Biology Annual Meeting, New Orleans LA, 2009.
  • Song, Michelle M., Elmore, Donald E., Sigman, Jeffrey A., Bruce, Lisa A., Pabon, Amanda, Glucksman, Marc J., and Wolfson, Adele J., "The 599-611 loop in thimet oligopeptidase modulates substrate selection via hydrogen bonding" American Society for Biochemistry and Molecular Biology Annual Meeting, San Diego CA, 2008.
  • Srikanthan, Meera A., Song, Michelle M., Bruce, Lisa A., Kua, L. H., Pabon, A., Glucksman, Marc J., Sigman, Jeffrey A., Elmore, Donald E., and Wolfson, Adele J., "Neuropeptide substrate specificity in thimet oligopeptidase is conferred by glycine residues residing in a loop region" American Society for Biochemistry and Molecular Biology Annual Meeting, Washington DC, 2007.
  • Randall D., Bruce B.A., Song M.M., Hayes G.A., Pabon, A., Glucksman M.J., Sigman J.A., and Wolfson A.J. "Loop region 599-611 determines substrate specificity in thimet oligopeptidase" American Society for Biochemistry and Molecular Biology Annual Meeting, San Francisco CA, 2006.
  • Stadelmann L.E., Leong R., Mann-Stadt C.F., Kannabiran D.R., Glucksman M.J., Sigman J.A., and Wolfson A.J. "Lowered flexibility in thimet oligopeptidase (EC3.4.24.15) leads to a change in substrate specificity" American Society for Biochemistry and Molecular Biology Annual Meeting, San Diego CA, 2005.
  • Joseph C.D., Sigman J.A., and Wolfson A.J. "Bradykinin hydrolysis by cyanobacterial cell extracts" American Chemical Society 225th National Meeting, New Orleans LA, 2003.
  • Patwa T.H., Sigman J.A., and Wolfson A.J. "Two-step denaturation of thimet oligopeptidase in urea" American Chemical Society 225th National Meeting, New Orleans LA, 2003.
  • Walsh S.T., Sigman J.A., Tablante A.V., Pabon A., Glucksman M.J., and Wolfson A.J. "Involvement of surface cysteines on thimet oligopeptidase activity and multimer formation" Experimental Biology, New Orleans LA, 2002.
  • Edwards S.R., Sigman J.A., and Wolfson A.J. "Binding of natural and artificial substrates to thimet oligopeptidase" Experimental Biology, New Orleans LA, 2002.
  • Sharky M.L., Kettenis H.A., Sigman J.A. and Wolfson A.J. "Involvement of surface cysteines in formation of multimers and inactivation of Endopeptidase EC 3.4.24.15" American Society for Biochemistry and Molececular Biology Annual Meeting, Orlando FL, 2001.
  • Sigman J.A., Walsh S.T., Shalev S.M., Kettenis H.A. and Wolfson A.J. "The role of Mn(II) and Ca(II) in enhancing the activity of 3.4.24.15" American Society for Biochemistry and Molececular Biology Annual Meeting, Orlando FL, 2001.
 
 

Professional Service

 

Presentation judge

  • ASBMB Undergraduate Student Poster Competition (2006, 2009, 2011, 2012)
 

Book reviews

  • Essentials of General, Organic and Biochemistry, by Denise Guinn and Rebecca Brewer, W.H. Freeman & Company (2011)
  • Student Companion to accompany Biochemistry, 6th edition and JMOL tutorial entitled Learning Protein and Nucleic Acid Structure with JMOL, W.H. Freeman & Company (2010)
  • Chapters 7 and 8 for the first edition of Guinn/Brewer's General, Organic, and Biochemistry: A One Semester Course, W.H. Freeman & Company (2006)
 

Workshop participation

 

ACS Research Symposium Organizer

  • Assisted Prof. Steve Bachofer in organizing The American Chemical Society Undergraduate Research Symposium held at Saint Mary’s College. Created an abstract submission form and several web pages for the symposium, collected and published the ~100 submitted presentation abstracts
  • Hosted a presentation session
 

Research article reviews

  • Journal of Biological Chemistry
  • Journal of the American Chemical Society
 
 

Institutional service (selected)

 

First Year Advising Task Force, member

2012-present

   

Chair, Technology Planning Steering Committee

2011-2012

   

Rank and Tenure Committee (R&T), member

2010-2012

   

Chair, Chemistry Department

2007-2009

   

Faculty Technology Group (FTG), chair

2008-2009

   

Faculty Technology Group (FTG), member

2006-2008, 2009-present

   

Technology Advisory Committee, FTG representative

2009-2012

   

Program Review Committee

2008-2009

   

Bookstore Committee, member

2004-present

   

Chemistry Skills Mastery Center (ChemSMC)

2008-2009

  • As chair of chemistry, I initiated and helped develop a study center for general chemistry students to develop study and problem-solving skills
 

Biochemistry program steering committee

2004-present

  • Developed course proposals for biophysical chemistry and advanced biochemistry courses
  • Co-wrote draft plan for biochemistry major at Saint Mary’s College
  • Advise the majority of the students in the major
 

Faculty adviser

 
  • Freshman cohort advisor
  • First Year Advising Cohort Instructor
  • Advisor to chemistry and biochemistry majors

2005-2007
2009-2011
2003-present

   
   

Awards, grants, & funded projects

 

Supplemental Awards grant Camille and Henry Dreyfus Foundation ($10,000)

2004

ASBMB travel grant ($500)

2010

SMC Faculty Development Fund Travel Award ($500-2,000)

2004-2012

SMC Faculty Development Fund Research Award ($1,100)

2012

 
 

Other appointments

   

Laboratory Technician

Ethyl Petroleum Additives, St. Louis, MO (1993-1994)

   

Analytical Laboratory Technician

Ethyl Petroleum Additives, St. Louis, MO (1991-1992)