Chemistry Department

Saint Mary's College of California



Research Publications

Bruce, Lisa.; Sigman, Jeffrey A.; Randall, Danica; Rodriguez, Scott; Song, Michelle; Dai, Amy; Elmore, Donald; Pabon, Amanda, Glucksman, M. J.; Wolfson, Adele J. Hydrogen bond residue positioning in the 599–611 loop of thimet oligopeptidase is required for substrate selection. FEBS. J. 275 (22), 5607-17 (2008).

Sigman, J. A.; Patwa, T.H.; Tablante, A.V.; Joseph, C.D.; Glucksman, M. J.; Wolfson, A. J. Flexibility in substrate recognition by thimet oligopeptidase as revealed by denaturation studies. Biochem. J. 388, 255-261 (2005).

Sigman, J. A.; Sharkey, M.L.; Walsh, S.T.; Pabon, A; Glucksman, M. J.; Wolfson, A. J. Involvement of Surface Cysteines in Activity and Multimer Formation of Thimet Oligopeptidase. Prot. Eng. 16, 623-628 (2003).

Sigman, J. A.; Edwards, S.R.; Pabon, A; Glucksman, M. J.; Wolfson, A. J. pH dependence studies provide insight into the structure and mechanism of thimet oligopeptidase (EC FEBS Lett. 545, 224-228 (2003).

Sigman J.A.; Kim H.K.; Zhao X.; Carey J.R.; Lu Y. The role of copper and protons in heme-copper oxidases: kinetic study of an engineered heme-copper center in myoglobin. Proc. Natl. Acad. Sci. U S A. 100, 3629-3634 (2003).

Perera, R., Sono, M., Sigman, J. A., Pfister, T. D., Lu, Y., and Dawson, J. H. Neutral thiol as a proximal ligand to ferrous heme iron: implications for heme proteins that lose cysteine thiolate ligation on reduction. Proc. Natl. Acad. Sci. U S A 100, 3641-3646 (2003).

Sigman, J. A.; Wang, X.; Lu, Y. Coupled oxidation of heme by myoglobin is mediated by exogenous peroxide. J. Am. Chem. Soc. 123, 6945 -6946 (2001).

Sigman, J. A.; Pond, A. E.; Dawson, J.H.; Lu, Y. From myoglobin to heme-copper oxidase: Design and engineering of a CuB center into sperm whale myoglobin. J. Am. Chem. Soc. 122, 8192 -8196 (2000).

Sigman, J. A.; Kwok, B.C.; Gengenbach, A.; Lu, Y. Design and creation of a Cu(II)-binding site in cytochrome c peroxidase that closely mimics the CuB-heme center in terminal oxidases. J. Am. Chem. Soc. 121, 8949-8950 (1999).

Sigman, J. A.; Pond, A. E.; Dawson, J.H.; Lu, Y. Engineering cytochrome c peroxidase into cytochrome P450: A proximal effect on heme-thiolate ligation. Biochemistry 38, 11122-11129 (1999).
Yeung, B. K.; Wang, X.; Sigman, J.A.; Petillo, P.A.; Lu, Y. Construction and characterization of a manganese-binding site in cytochrome c  peroxidase: towards a novel manganese peroxidase. Chem. Biol. 4, 215-221 (1997).

Bold signifies undergraduate co-authors

cFP, a potent inhibitor of TOP
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    Teaching Publications

Sigman, J. A. Thermodynamic properties of peptide folding. Biochemistry and Molecular Biology Education 32, 265-268 (2004).